Microbial Identification Systems (MIDI)
We offer here a group of product to help you identify bacterias,
The major component is Sherlock® : the software controling the GC, communicating with chemstation and analysing the data.
Fatty acid methyl ester analysis by gas chromatography (GC-FAME) has been used for over 50 years to identify microbes in environmental and clinical settings. FAME identification is a dynamic test of the organism. It is specific to growth conditions, including the media and temperature used. Different libraries are available for different growth conditions and custom libraries can be created based on the customer's unique environment. In addition to identification, FAME analysis is often sensitive enough to give strain level information for a sample.
The Sherlock Microbial Identification System (MIS) was launched in 1985 and represents a rapid, accurate and comprehensive solution for the identification of over 1,500 microbial species by FAME analysis. The Sherlock MIS is recognized by the U.S. CDC for the identification of aerobic bacteria and is AOAC INTERNATIONAL cleared for the identification of Bacillus anthracis, the anthrax pathogen. The Sherlock MIS was created by microbiologists for microbiologists, so no GC experience is necessary.
Different procedures can be used to extract the fatty acid methyl esters (FAME) from the bacteria. MIDI has developped faster, simpler methods over the years
Ideal for Aerobe bacterias
Instant Anaerobe FAME
Developed to Anaerobe bacterias
More efficient for gram negative bacterias
Identifies microorganisms by their fatty acid profile
Libraries Identify (Aerobes, Anaerobes, Yeast, Bioterrorism Agents)
Rapid Aerobe Analysis
Instant Fame Analysis
DNA sequence analysis
The system uses gas chromatographs, MIDI microbial databases and MIDI pattern recognition software to identify each strain
||Sherlock® DNA offers an array of features for bacterial/fungal identification and analysis.
• 16S rRNA DNA-based microbial identification for bacteria.
• 28S rRNA DNA-based microbial identification for fungi and yeast.
• Validated libraries available for 500 base-pair and full-gene 16S bacterial identification,
as well as 28S fungi/yeast identification.
• Full integration with Sherlock's Microbial Identification System for fatty acid methyl
ester (FAME) analysis of bacterial and yeast/fungal samples.
• Combined reporting of DNA-FAME results.
• DNA analysis tools for direct comparison of DNA samples.
• Custom library development tools for creating additional libraries.
The Sherlock DNA system begins where the sequencer leaves off: with a textual consensus
sequence. The advantage of this approach is that Sherlock DNA can be used with any sequencer
from any vendor.
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